Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey
Identifieur interne : 001665 ( Main/Exploration ); précédent : 001664; suivant : 001666Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey
Auteurs : Chris Gardiner [Royaume-Uni] ; Dolores Di Vizio [États-Unis] ; Susmita Sahoo [États-Unis] ; Clotilde Théry [France] ; Kenneth W. Witwer [États-Unis] ; Marca Wauben [Pays-Bas] ; Andrew F. Hill [Australie]Source :
- Journal of Extracellular Vesicles [ 2001-3078 ] ; 2016.
Abstract
Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed.
Url:
DOI: 10.3402/jev.v5.32945
PubMed: 27802845
PubMed Central: 5090131
Affiliations:
- Australie, France, Pays-Bas, Royaume-Uni, États-Unis
- Angleterre, Californie, Grand Londres, Maryland, Utrecht (province), État de New York, Île-de-France
- Baltimore, Londres, Paris, Utrecht
- University College de Londres, Université d'Utrecht
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Witwer</name><affiliation wicri:level="3"><nlm:aff id="AF0005">Johns Hopkins University School of Medicine, Baltimore, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Johns Hopkins University School of Medicine, Baltimore</wicri:regionArea><placeName><settlement type="city">Baltimore</settlement><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Wauben, Marca" sort="Wauben, Marca" uniqKey="Wauben M" first="Marca" last="Wauben">Marca Wauben</name><affiliation wicri:level="4"><nlm:aff id="AF0006">Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region><settlement type="city">Utrecht</settlement></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Hill, Andrew F" sort="Hill, Andrew F" uniqKey="Hill A" first="Andrew F." last="Hill">Andrew F. Hill</name><affiliation wicri:level="1"><nlm:aff id="AF0007">Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora</wicri:regionArea><wicri:noRegion>Bundoora</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of Extracellular Vesicles</title><idno type="eISSN">2001-3078</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Extracellular vesicles (EVs) represent an important mode of intercellular communication. Research in this field has grown rapidly in the last few years, and there is a plethora of techniques for the isolation and characterization of EVs, many of which are poorly standardized. EVs are heterogeneous in size, origin and molecular constituents, with considerable overlap in size and phenotype between different populations of EVs. Little is known about current practices for the isolation, purification and characterization of EVs. We report here the first large, detailed survey of current worldwide practices for the isolation and characterization of EVs. Conditioned cell culture media was the most widely used material (83%). Ultracentrifugation remains the most commonly used isolation method (81%) with 59% of respondents use a combination of methods. Only 9% of respondents used only 1 characterization method, with others using 2 or more methods. Sample volume, sample type and downstream application all influenced the isolation and characterization techniques employed.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Raposo, G" uniqKey="Raposo G">G Raposo</name></author><author><name sortKey="Stoorvogel, W" uniqKey="Stoorvogel W">W Stoorvogel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yanez Mo, M" uniqKey="Yanez Mo M">M Yanez-Mo</name></author><author><name sortKey="Siljander, Pr" uniqKey="Siljander P">PR Siljander</name></author><author><name sortKey="Andreu, Z" uniqKey="Andreu Z">Z Andreu</name></author><author><name sortKey="Zavec, Ab" uniqKey="Zavec A">AB Zavec</name></author><author><name sortKey="Borras, Fe" uniqKey="Borras F">FE Borras</name></author><author><name sortKey="Buzas, Ei" uniqKey="Buzas E">EI Buzas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Pol, E" uniqKey="Van Der Pol E">E van der Pol</name></author><author><name sortKey="Boing, An" uniqKey="Boing A">AN Böing</name></author><author><name sortKey="Gool, El" uniqKey="Gool E">EL Gool</name></author><author><name sortKey="Nieuwland, R" uniqKey="Nieuwland R">R Nieuwland</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gould, Sj" uniqKey="Gould S">SJ Gould</name></author><author><name sortKey="Raposo, G" uniqKey="Raposo G">G Raposo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lotvall, J" uniqKey="Lotvall J">J Lotvall</name></author><author><name sortKey="Hill, Af" uniqKey="Hill A">AF Hill</name></author><author><name sortKey="Hochberg, F" uniqKey="Hochberg F">F Hochberg</name></author><author><name sortKey="Buzas, Ei" uniqKey="Buzas E">EI Buzas</name></author><author><name sortKey="Di Vizio, D" uniqKey="Di Vizio D">D Di Vizio</name></author><author><name sortKey="Gardiner, C" uniqKey="Gardiner C">C Gardiner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Deun, J" uniqKey="Van Deun J">J Van Deun</name></author><author><name sortKey="Mestdagh, P" uniqKey="Mestdagh P">P Mestdagh</name></author><author><name sortKey="Sormunen, R" uniqKey="Sormunen R">R Sormunen</name></author><author><name sortKey="Cocquyt, V" uniqKey="Cocquyt V">V Cocquyt</name></author><author><name sortKey="Vermaelen, K" uniqKey="Vermaelen K">K Vermaelen</name></author><author><name sortKey="Vandesompele, J" uniqKey="Vandesompele J">J Vandesompele</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hill, Af" uniqKey="Hill A">AF Hill</name></author><author><name sortKey="Pegtel, Dm" uniqKey="Pegtel D">DM Pegtel</name></author><author><name sortKey="Lambertz, U" uniqKey="Lambertz U">U Lambertz</name></author><author><name sortKey="Leonardi, T" uniqKey="Leonardi T">T Leonardi</name></author><author><name sortKey="O Driscoll, L" uniqKey="O Driscoll L">L O'Driscoll</name></author><author><name sortKey="Pluchino, S" uniqKey="Pluchino S">S Pluchino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lener, T" uniqKey="Lener T">T Lener</name></author><author><name sortKey="Gimona, M" uniqKey="Gimona M">M Gimona</name></author><author><name sortKey="Aigner, L" uniqKey="Aigner L">L Aigner</name></author><author><name sortKey="Borger, V" uniqKey="Borger V">V Borger</name></author><author><name sortKey="Buzas, E" uniqKey="Buzas E">E Buzas</name></author><author><name sortKey="Camussi, G" uniqKey="Camussi G">G Camussi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Webber, J" uniqKey="Webber J">J Webber</name></author><author><name sortKey="Clayton, A" uniqKey="Clayton A">A Clayton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Erdbrugger, U" uniqKey="Erdbrugger U">U Erdbrügger</name></author><author><name sortKey="Rudy, Ck" uniqKey="Rudy C">CK Rudy</name></author><author><name sortKey="E Etter, M" uniqKey="E Etter M">M E. 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Mattson</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li><li>France</li><li>Pays-Bas</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Californie</li><li>Grand Londres</li><li>Maryland</li><li>Utrecht (province)</li><li>État de New York</li><li>Île-de-France</li></region><settlement><li>Baltimore</li><li>Londres</li><li>Paris</li><li>Utrecht</li></settlement><orgName><li>University College de Londres</li><li>Université d'Utrecht</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Gardiner, Chris" sort="Gardiner, Chris" uniqKey="Gardiner C" first="Chris" last="Gardiner">Chris Gardiner</name></region></country><country name="États-Unis"><region name="Californie"><name sortKey="Vizio, Dolores Di" sort="Vizio, Dolores Di" uniqKey="Vizio D" first="Dolores Di" last="Vizio">Dolores Di Vizio</name></region><name sortKey="Sahoo, Susmita" sort="Sahoo, Susmita" uniqKey="Sahoo S" first="Susmita" last="Sahoo">Susmita Sahoo</name><name sortKey="Witwer, Kenneth W" sort="Witwer, Kenneth W" uniqKey="Witwer K" first="Kenneth W." last="Witwer">Kenneth W. Witwer</name></country><country name="France"><region name="Île-de-France"><name sortKey="Thery, Clotilde" sort="Thery, Clotilde" uniqKey="Thery C" first="Clotilde" last="Théry">Clotilde Théry</name></region></country><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Wauben, Marca" sort="Wauben, Marca" uniqKey="Wauben M" first="Marca" last="Wauben">Marca Wauben</name></region></country><country name="Australie"><noRegion><name sortKey="Hill, Andrew F" sort="Hill, Andrew F" uniqKey="Hill A" first="Andrew F." last="Hill">Andrew F. Hill</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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